Future investigations of the Martian subsurface are expected to focus on the detection of extant life. Past geophysical work suggested that the Kobuk Dunes in Northern Alaska contain a perched liquid water layer 4 m below the dune crests, which is located above the mean annual water table approximately coincident with the interdune surfaces at ~70 m above mean sea level. The reason why this perched water exists remains unknown; however, it’s presence represents an important analog for understanding the astrobiological potential of the Martian subsurface in aeolian environments. Our March and upcoming September sampling events will enable us to constrain how seasonal processes and changes in habitability within the Kobuk dunes influence the distribution of life and life related indicators and how this may aid in our search for life in subterranean Mars.