The use of an RT (Reflectance-Transmittance) sphere with the high-resolution NaturaSpec Plus spectroradiometer offers a major improvement in measuring leaf reflectance for environmental and agricultural studies. The RT Sphere accessory captures total hemispherical reflectance, reducing errors caused by leaf surface texture, angle, and lighting direction. This leads to more accurate and consistent data. Reflectance and transmittance measurements help researchers understand plant health, nutrients, and structure. When combined with EZ-ID spectral matching software, users can better analyze plant spectra and track changes in real time. This study looks at different types of leaves to identify the limitations of standard leaf clips, especially for waxy or uniquely shaped leaves. We compare the strengths and weaknesses of the RT sphere and traditional leaf clip to guide users in choosing the right tool for their research. Understanding when and how to use each method supports better decisions in precision agriculture and environmental monitoring.