Thanks to their intrinsic properties and locations, the Galilean Moons of Jupiter provide us with unique opportunities to learn about the role of moons in a planetary magnetosphere. Jupiter is connected to these moons not just gravitationally, but also magnetically through magnetic flux tubes along magnetic field lines. Inside these flux tubes, both Jupiter and the moons affect the plasma environment and the electromagnetic field, as opposed to just influences from Jupiter outside the tubes. In this study, we examine how the combined effects from both Jupiter and a given moon vary inside its flux tubes, and investigate how these effects depend on magnetic latitude and magnetic local time. Our findings provide valuable insights into the role of the Galilean moons in shaping the Jovian inner and middle magnetosphere.