Green spaces decrease as cities grow, and plant and soil communities frequently deteriorate. However, even the smallest urban areas, such as crevices in sidewalks and roads, can support life and offer unexpected ecological advantages. In Nashville, Tennessee-USA, we investigated urban crevice microhabitats, focusing on the soils beneath pavement crevices as well as the plants that grow there. We collected soil samples from a switchgrass cropland in the city, adjacent roadsides, and crevices with and without vegetation. Only 12 of the 34 plant species that were identified growing in pavement cracks were native, suggesting that non-native species dominate in these areas. Compared to the other soils, crevice soils were significantly lower in moisture but higher in temperature, pH, and ion levels. Also, crevice soils supported unique biological activity and retained more carbon, nitrogen, and microbial biomass in spite of the harsh conditions than the other soil types. Based on these results, pavement crevices, which are frequently disregarded, may serve as urban havens for resilient plant species and soil life. We continue to study how these habitats sustain microbial diversity and respond to pollution. Overall, crevice microhabitats can significantly contribute to biodiversity conservation, carbon storage, urban climate resilience, and sustainable city planning.