Antimicrobial resistance (AMR) promotes microbiome adaptation to stress, but the contribution of human land use history to antibiotic resistance genes (ARG) evolution is not well characterized. Urban soils have undergone substantial land development, resulting in spatially heterogeneous metal deposition that may drive AMR evolution as microbes adapt to metal stress. Our overall objective is to elucidate the human socioecological drivers of soil microbiome AMR functions within Chicago, Illinois. We hypothesize that spatial heterogeneity in metal contamination will correlate to parallel patterns in ARG distribution within the Chicago soil microbiome and that these patterns are influenced by social determinants of health that influence metal deposition. We sequenced soil cores (N = 300) collected throughout Chicago, Illinois, using Illumina shotgun metagenomics to characterize the microbiome and the diversity of ARGs, incorporating microbial ARG data with metal analyses (Cu, Zn, Pb, Co, Cd, etc.) and social determinants of health (i.e. traffic intensity, urban tree greenness, etc.), to identify signatures of land use within the soil microbiome. Herein, we present the preliminary metagenomics data in efforts to expand our understanding of the dynamics between soil metal contamination and microbial AMR processes to support urban environmental health.